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陸上短距離運動選手における心拍変動解析による自律神経機能評価と白血球分画の検討

Evaluation of autonomic nervous system by heart rate variability and differential count of leukocytes in athletes

Key words:心拍変動解析 heart rate variability、コンディショニング conditioning、白血球分画 differential count of leukocytes

末武信宏1.2、森田有紀子2、小林弘幸1、鈴木大地3

1.順天堂大学大学院医学研究科 病院管理学
〒113-8421 東京都文京区本郷2丁目1番1号
FAX:03-3813-3622
TEL:03-3813-3111 

2.さかえクリニック
〒460-0003 愛知県名古屋市中区錦3-5-21 錦HOTEIビル 2階
TEL: 052-953-9676
FAX: 052-953-7810

3. 順天堂大学スポーツ健康科学部スポーツ科学科
〒270-1695 千葉県印旛郡印旛村平賀学園台1-1
TEL: 0476-98-1001
FAX: 0476-98-1011

国内トップアスリートである陸上短距離運動選手の短時間全力運動前後の身体的状態を、自律神経機能検査、血液生化学検査、白血球分画検査、乳酸値測定を行い得られた結果から選手のコンディショニング定量評価法を検討した。また、運動前、運動10分後のデータに統計学的分析を加え回復期における陸上短距離運動選手の自律神経機能を検討した。白血球分画において運動前後に変化する白血球の割合の増減から好中球と交感神経、リンパ球と副交感神経の関連1)の可能性も確認し、この実験結果から陸上短距離運動選手において自律神経機能のパラメータ、白血球分画が有意に短時間全力運動前後で変化することが分かった。


Ⅰ.はじめに
アスリートにとって、常に良いコンディションを保ってトレーニングを行うことは大変重要なことである。また、アスリートは日常的に高強度・長時間・高頻度のトレーニングを実施している。このため激しいトレーニングの繰り返しが身体的疲労に併行して精神的疲労を生じさせることになる。トレーニングを行う上でコンディショニングは重要なファクターでありオーバートレーニング症候群2)発症を防止するうえにも必ず考慮すべきことである。しかし、これまでコンディショニングとして血中乳酸値の測定による身体的疲労評価、POMS(Profile Of Mood State)を用いた精神的疲労評価を行われてきたが十分な評価が必ずしも得られていない。アスリートのコンデショニングを評価する手法として、今回我々は、心拍変動解析3)による自律神経機能定量評価4)5)を提案する。これまで行われてきたコンディショニングの検査と比較して、より具体的にアスリートの身体情報を得ることが可能となった6)。心臓の拍動は、自律神経によってリズムの調整がされており、自律神経は、交感神経と副交感神経とからなり、両者は拮抗的に働く。交感神経は、緊張や興奮など何らかのストレス状態になったときに優位となり、反対に副交感神経が優位のときは、心臓の働きは抑制される。したがって、心拍数やRR間隔変動(心拍変動)を測定することにより自律神経の影響、さらにそれを変化させる原因となっている精神的な安定度やストレスの程度などを類推することができる(心拍変動解析)7)8)。また同時にこれまでアスリートのコンディショニング検査として普及している 血液生化学検査(Aspartate Amino Transferase (AST)、Alanine Amino Transferase(ALT)、Lactate Dehydrogenase (LDH)、Creatine Kinase (CK)、腎機能及び蛋白質代謝の変化を把握するためにBlood Urea Nitrogen (BUN)、)糖代謝状況を把握するために血中乳酸値、最近、自律神経機能と関係が指摘されている白血球分画(好中球、リンパ球、単球、好酸球、好塩基球)を検査した9)。安保は白血球が自律神経支配を受けていることを報告している10)。即ち、白血球主要成分である顆粒球ではアドレナリン受容体が交感神経刺激により、またリンパ球ではアセチルコリン受容体が副交感神経刺激で活性化する11)。白血球には生体防御活動を行う二大防御細胞が存在し、外界から侵入する細菌や異物を貪食する顆粒球やマクロファージ等の食細胞とウイルスや異種タンパクの侵入に対し、抗原―抗体反応や細胞障害反応にて攻撃するリンパ球である。今回、我々は、顆粒球の代表的な好中球とリンパ球の比率に注目した。自律神経パラメータの推移と白血球とリンパ球の比率を陸上短距離運動選手の全力もも上げ運動前後で測定することにより、陸上短距離運動選手の肉体的・精神的疲労の新しい指標となる評価法を探り、コンディショニングの評価指標の可能性についての検討と提案を試みた。
Ⅱ.対象と方法
Ⅱー1.対象
研究対象は、20歳代の現役陸上短距離運動選手 5名(27±1.4歳)で、全員陸上県選手権大会入賞以上の実績(全員2009年度の自己最高記録が日本ランキング20以内の選手であり1名は女子、他4名は男子)を持つ陸上短距離運動選手 5名とした。全被験者の1週間の練習スケジュールは週1日の休養、週5日間の1日3時間以上のサーキットトレーニング、ウエイトトレーニングを行って日曜日は主に競技会、記録会への出場である。被験者全員1週間に5日以上、1日3時間以上のフィジカルトレーニングを行っている者であった。また、本研究は全ての被験者へ研究の目的及び内容、実験の具体的な運動など参加することによる身体的・精神的負担をできる限り詳細かつ、具体的に説明した。さらに、競技会への影響がないように実験日時を調整し、参加することに同意が得られた者を対象とした。被験者は実験前には軽いウォームアップを普段通りに行い、体調不良がないことを確認した。被験者全員は非喫煙者であり,高血圧,高脂血症,心血管疾病と糖尿病などの疾患に躍っておらず,心血管機能に影響する薬剤、また睡眠薬や抗不安薬など精神的な影響を与える薬剤を服用していない者を対象とした。実験当日、体温37℃以内で強い疲労感を感じておらず、前日の睡眠時間は6時間以上でアルコールを摂取していない者を対象とした。測定前 3時間以上 被験者には飲食を禁止し、測定前に排尿を行い膀胱内に尿が貯留していないことを条件とした。運動前は坐位にて20分間以上の安静状態を確認して測定を行った。この時の呼吸数は毎分13回程度に保つように規定した。
Ⅱ-2.方法
心拍変動解析:心拍数やRR間隔変動(心拍変動)を測定することにより自律神経の影響、さらにそれを変化させる原因となっている精神的な安定度やストレスの程度などを類推することができる。RR間隔とは、心電図の波形で一番大きくスパイク状に出るR波と次のR波までの間隔のことである。このRR間隔変動をスペクトル変換したときの高周波成分(>0.15Hz)を「HF」、低周波成分(0.04~0.15Hz)を「LF」と呼び、HF成分は、心臓を支配している自律神経の副交感神経の活動のみを反映するとされている。また、HF成分は通常、呼吸性洞性不整脈に対応する。したがって、その周波数は呼吸の周波数に等しい。一方、LF成分は、交感神経と副交感神経の両方の活動を反映するとされている。そのため、HF成分とLF成分の比率が、交感神経および副交感神経の活動指標となるとされている。一般に、LF/HF比を交感神経の活動性の指標とし、HFを副交感神経の活動性の指標とする。周波数領域解析のパラメータは、高速フーリエ変換法(FFT)により計算されたパワースペクトルから算出される12)。この実験では周波数領域分析パラメータ であるvery low frequency (VLF)、high frequency (HF)、 low frequency (LF)、 LF/HF比、 及びTotal Powerを測定項目とする。全力もも上げ運動前と運動10分後に血液生化学検査としてAST、ALT、LDH、CK、血糖、BUN、そして白血球分画、血中乳酸値を検査した。この場合、HF、LFはHF Norm(HF補正値)、LH Norm(LH補正値)を示し、補正値を用いて統計的検討を行う。
HF Norm=HF/(Total Power-VLF)×100
LF Norm=LF/(Total Power-VLF)×100
この補正はVLFの変化による影響を最小限に抑え、それぞれ副交感神経活動、交感神経活動による変化を強調するものである。VLFは虚血性心疾患の患者の予後を予測する上で重要な因子と近年注目を集めている13)。しかし、VLFは交感神経機能の非常にゆっくりしたメカニズム全体の活動を示すものとされているが生理学的メカニズムは不明の点も多い。よって今回の測定では取り除くべき成分と考え補正値の数値で統計学的検討を行うものとする。
運動前、被験者は静かな部屋で安静坐位時に ヨーロッパ心臓病学会と北米ペーシング電気生理学会(The European Society of Cardiology and The North American Society of Pacing and Electrophysiology)によって提唱された短時間心拍変動解析のアルゴリズムを採用しているソフトウエアを組み込んだ 心拍変動解析システム Heart Rhythm Scanner(Biocom社)を使用して測定を行った。右耳垂部にPhotoplethysmography(PPG)モニターを装着し5分間の心拍変動を測定し解析を行った。運動後も同じ部屋において同じ椅子で安静坐位にて測定を行った。PPGモニターはBiocom社製 HRM-02脈派センサーを使用した。PPGモニターは、光源とフォトセル(光をエネルギーに変換する)からなる変換機上の両方に耳垂部が接するように置くことによって機能し赤外線の光線はフォトセルに向かって発射される。耳垂部の中の血管の血液流量変化は心拍間隔に関連しているためPPGモニターにより脈波が測定可能となり心拍変動のデータを得ることができる。時間帯は心拍変動変化が少ない午後0:00~6:00の間に室温24℃、湿度60%の室内で定位置にて短時間全力運動として全力もも上げ運動 を 1分間2セット 1分間のインターバルで実施した。全力もも上げ運動中は担当トレーナーが、かけ声をかけ途中でもも上げ運動のスピードを緩めないよう、全力でのもも上げ運動維持をしっかり管理して被験者が疲労困憊に至るよう実施した。1分間のインターバル中は立位にて安静を保った。運動終了後は仰臥位にて10分間の安静回復を行った。運動10分後に運動前に測定、採血を行った安静坐位の状態で運動10分後の自律神経機能測定及び定量評価を行った。運動後の自律神経機能測定の際には、できる限り呼吸を一定に保つように指示した。運動前後、同一側の肘正中皮静脈より採血を行った。採血した血液検体は、株式会社 ファルコバイオシステムズへ検査項目の測定を依頼した。また、運動直後に主観的運動強度を聞き取り調査した。実験における運動後のすべての検査・測定は室温24℃、湿度60%の部屋にて安静坐位で行った。
血液生化学検査、白血球分画、乳酸値:運動実施に伴うエネルギー代謝の状況を観察するためBlood glucose (BG)、筋組織の炎症や損傷状況を把握する目的でAspartate Amino Transferase (AST)、Alanine Amino Transferase(ALT)、Lactate Dehydrogenase (LDH)、Creatine Kinase (CK)、腎機能及び蛋白質代謝の変化を把握するためにBlood Urea Nitrogen (BUN)、糖代謝状況を把握するために血中乳酸値、自律神経との関係が考えられる白血球分画(好中球、リンパ球)を検査した。
Ⅱ-3.統計処理
実験で得られたデータは運動前後の変化を、対応のあるt検定で解析を行い、p<0.05を有意差あり、と判定した。解析ソフトは、PASW Statistics 17.0 (SPSS Inc.) を使用した。
Ⅲ.結果
Ⅲ-1.心拍変動解析  (図1~図5)
全被験者の安静時における、自律神経機能のパラメーター Total power 、very low frequency(VLF)、high frequency (HF)、low frequency (LF)、LF/HF比はすべて基準値内であった。
安静時の5人の陸上短距離運動選手の全力もも上げ運動前と運動10分後の Total Power、VLF、LF Norm、HFNorm、LF/HF比の平均値の比較である。Total power(p<0.05)、LF(p<0.01)、LF/HF比(p<0.05)はいずれも有意に減少していた。HFは有意に増加(p<0.05)していた。VLFは有意ではない(p<0.1)が減少傾向が認められた。
Ⅲ-2.血液生化学検査、乳酸値
運動前後の血液生化学検査、乳酸値はすべて基準値内であった。Creatine Kinase (CK)を除いて血液生化学検査の運動前後の値の変化の有意差は認められなかった。短時間全力運動10分後に、Creatine Kinase (CK)、血中乳酸値、は有意に増加した14)(p<0.01)。
Ⅲ-3.白血球分画  (図6~図7)
白血球分画での好中球、リンパ球の比率はすべて基準値内であった。5人の陸上短距離運動選手の全力もも上げ運動前と運動10分後の好中球、リンパ球比率の平均値の比較である。好中球比率は運動後に有意に低下(p<0.01)、リンパ球比率は運動後に有意に増加(p<0.01)した。
Ⅳ.考察
多くのアスリートの他覚的コンディショニング指標を測る目的でとして血液検査、心理テスト、自律神経機能検査などが行われている。アスリートにとって自律神経が運動時に重要な役割を果たすことは明確である。これまでも様々なアスリートにおける心拍変動解析による自律神経機能評価の報告がみられる15)。しかし、最近では自律神経と白血球の関連が報告されているにも関わらずスポーツ分野においてこの関連が検討されることはこれまでほとんどなかった。運動後に白血球が増加することは知られている。運動前後の白血球分画の変化の報告はある16)が自律神経との関連を検討した報告はなかった。今回の我々の研究ではアスリートの運動前後の自律神経機能定量評価に加えて白血球分画にも注目し検討を加えた。
今回の実験では日常的に高頻度、高強度の運動を行っているアスリートを対象にしたため疲労困憊の状態に被験者を誘導する目的で全力もも上げ運動を運動負荷試験として選択した。全力もも上げ運動後は著明に血中乳酸値の上昇を認めており本研究の全力運動後は他覚的にも被験者が疲労困憊の状態になったことを示すものであった。自律神経のTotal powerは運動後有意に減少しているがこれは、大きな身体的ストレスにより一時的に自律神経機能に抑制がかかったと考えられる。慢性疲労的な状態であるオーバートレーニング症候群でも自律神経機能低下がすでに報告されており、同様の状態が想定されTotal powerが低下した状態でのトレーニングを行う場合、注意が必要である。本研究においてLFが運動後に有意に減少しているのは、交感神経活性は最大運動により亢進するが、心臓の圧受容体調整が抑制され、その結果LFは減少する。つまり、LFは交感神経成分のみならず圧受容体反射にも依存しているためと考えられる。短時間全力運動により急激な心拍数増加により交感神経緊張状態を改善するため運動10分後に副交感神経活動が増加すると考えられる。今回の運動後の測定は回復期と考えられる短時間全力運動後10分後に行った。アスリートにとって全力運動によって急激に上昇した心拍数を低下させることは大きな意味を持つ。急激に変化した身体状況を一定に保持する自律神経機能の変化は非運動選手に比較した場合、差がでると予想される。今回の実験対象はトップアスリートである陸上短距離運動選手を被験者としたが非運動選手の場合は、一般的に副交感神経活動がアスリートに比較して低いことが指摘されている。このため短時間全力運動後の自律神経機能のパラメーターは異なる結果が予想される。また、今回の実験で3日間に及ぶ大学陸上競技大会に主力選手として出場し精神的にも肉体的にも疲労した状態で参加した陸上短距離運動選手1名が残念ながら研究被験者の対象外となった。この陸上短距離運動選手は、安静時の自律神経機能結果が標準値を下回り自覚症状もあったが実験前に申告せず本実験の被験者となった。この運動選手の検査結果では副交感神経活動が運動後も低下した状態が継続していた。このことは、オーバートレーニング症候群のアスリートへさらに負荷をかけるトレーニングは自律神経機能の回復を遅らせる可能性があると考えられる。アスリートは非運動者に比較して安静時に副交感神経緊張が有意に高いとこれまでも報告されているが、運動後の回復も副交感神経緊張によることが大きいと考えられる17)。一般的に心拍変動による心臓自律神経活動評価の原理は、走っている車の速度の変化からアクセルとブレーキの操作を推定することに例えられる。車の速度の変化は心拍変動に、アクセルとブレーキの操作は心臓に対する交感神経活動と副交感神経活動に対応する。アスリートにとって急激に上昇した心拍数を安静時に急激に調整するために副交感神経緊張状態を高くすることは意味がある。今回の実験で交感神経の活動指標であるLF/HF比が全力もも上げ運動10分後に低下し副交感神経の活動指標であるHFが運動10分後に上昇するという結果が得られたが、この結果は白血球分画における好中球とリンパ球の割合の増減と一致している。自律神経と白血球の関係が指摘されているが、安保が報告しているように交感神経は顆粒球、副交感神経はリンパ球との活動が影響を及ぼしている可能性がある18)。アスリートにおいて、短時間全力運動前後に、有意に心拍変動解析のパラメーターの増減、白血球分画の変化を認めたことは心拍変動解析による自律神経機能定量評価や白血球分画のチェックはアスリートのコンディショニングやアスリートの身体能力評価指標となりうる可能性がある。特に好中球とリンパ球比率の変化は自律神経機能同様、有用な指標になる可能性がある。
今回の実験の被験者は陸上短距離競技におけるトップアスリートであり運動後の自律神経機能の回復能力に優れていることが予想された。しかし、非運動選手の場合は、運動後の回復期の自律神経の状態がアスリートとは異なることが予想される。本研究の実験日は、陸上短距離運動選手の競技会シーズン中であり、日本ランキング上位の国内のトップ選手の多くを被験者とすることには困難な状況であった。実際に実験を承諾した数名の被験者も日常のトレーニングによる疲労から実験をキャンセルした。身体へ大きな負担が予想される実験のため倫理的にも競技会への参加や選手個人のトレーニングを妨げない配慮を行った結果、当初予定していた被験者数を下回ることになった。今回の実験では被験者が陸上短距離運動選手であったが異なる競技の運動選手の場合、違う結果が得られる可能性もあるが、陸上短距離運動選手は最も基本運動を行うアスリートであり急激な心拍数の変動が得られることで被験者に選定した。また、今回の被験者では、運動前後のパラメーターの変化に個体間のばらつきがあり、今後慎重に症例を増やしての更なる検討を要す。コントロール群との比較、運動強度を変えての解析や、運動後に介入をおこなった場合の変化なども検討したい。
参考文献

 
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      Evaluation of autonomic nervous system by heart ratevariability and differential count of leukocytes inathletes
      Nobuhiro Suetake1,2*, Yukiko Morita2, Daichi Suzuki3, Keiko Lee1, Hiroyuki Kobayashi1

      1Department of Hospital Administration Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan
      2Sakae Clinic, Nishiki Hotei Bldg.2F, Nishiki, Naka-ku, Nagoya-shi, Aichi, Japan; *Corresponding Author: nobu666@d1.dion.ne.jp
      3Department of Sports Science Juntendo University Graduate School of Health and Sports Science, Hiraga gakuendai, Inba-mura,
      Inba-gun, Chiba, Japan
       
      Received 22 May 2010; revised 15 June 2010; accepted 2 July 2010.
       
      ABSTRACT
      Top Japanese sprinters were evaluated for their physical condition, autonomic function, blood chemistry, differential leukocyte count and blood lactate level before and after short, maximal exercise to explore methods of quantifying their conditioning level. Statistical analysis of data obtained before and 10 min after exercise were used to estimate the athletes’ autonomic capacity during recovery. Pre and post exercise variances in differential leukocyte count revealed strong correlations between neutrophil and sympathetic activity, and lymphocyte and parasympathetic
      activity. The results of the study demonstrated significant alterations in autonomic parameters and differential white blood cell count in response to maximal exercise.

      Keywords: Heart Rate Variability; Conditioning;
      Differential Count of Leukocytes

      1. INTRODUCTION
      It is of paramount importance for high-performance athletes to consistently train in a manner that preserves their optimal conditioning level. Athletes regularly train at
      high intensity and volume for extended periods such that an imbalance between excessive workload and inadequate recovery renders them susceptible to mental and
      physical depletion. Therefore, athletes` fitness level is an important factor in designing training programs and for preventing overtraining syndrome [1]. However, previous
      attempts to portray the blood lactate level and Profile of Mood State (POMS) as respective indicators of physical and mental fatigue have not been entirely conclusive.
      For this study, heart rate variability (HRV) analysis [2] has been selected to quantitatively evaluate autonomic capacity [3,4] for its greater accuracy in estimating
      physical fitness than earlier methods [5]. Cardiac rhythm is modulated by the two limbs of the autonomic nervous system (ANS), the sympathetic and parasympathetic,
      which exert antagonistic effects. Sympathetic dominance occurs during stressful conditions such as nervousness and excitement, whereas a shift in favor of vagal
      modulation calms the heart rate. Accordingly, heart rate and RR interval fluctuation (HRV) permit analogical inference of autonomic influence as well as the effects
      of mental state and stress level on sympathetic and vagal outputs (HRV analysis) [6,7].
      Abo has previously reported that leukocytes are under autonomic control [8]. More specifically, sympathetic and parasympathetic stimulations have been shown to
      activate adrenalin receptors on granulocytes, the most prolific leukocytes, and acetylcholine receptors on lymphocytes, respectively [9]. Leukocytes have two major
      biological defense activities. Granulocytes and macrophages incite phagocytic activities against foreign bacteria and materials, whereas lymphocytes respond to viral
      and abnormal protein infiltrations by mediating antigenantibody production and attacking cellular damage.
      In this study we have focused on the proportion of neutrophil, the representative granulocyte, and lymphocyte subpopulations. In short, we have explored whether
      the alterations in autonomic parameters and granulocyte/ lymphocyte ratio serve as novel markers of physical and mental exhaustion in sprinters after maximal high
      knee lifts, and also act as potential indicators of athletes’ fitness level.

      2. MATERIALS AND METHODS
      2.1. Subjects
      Five elite sprinters in their twenties (mean age 27 ± 1.4)
      with qualifications or titles in the Prefectural Track and Field Championships participated in the study (1 female and 4 male sprinters with personal bests ranking among the top 20 in 2009). Weekly training program for the athletes consisted of daily circuit and weight training for >/= 3 hr/day for more than 5 days a week, punctuated
      with a day of rest and participation in competitive and time trials on Sundays. All participants routinely engaged in physical exercise for >/= 3 hr/day for at least 5
      times per week. Subjects were fully informed about the objectives, specific test procedures, and mental and physical burden of participating in the study. Test times
      were scheduled to avoid conflict with competitions and to prevent carryover effects on athletic performance.
      Eligible participants provided full consent before enrolling in the study. Subjects performed a light warm-up before the test exercise to assess their condition. All study
      participants were nonsmokers and had no history of hypertension, hyperlipidemia, cardiovascular disease, or diabetes. None of the athletes were taking cardiovascular
      medications and psychiatric agents such as sleeping pills and anxiolytic drugs. On the day of the test, subjects must have had a body temperature of >/= 37℃, absence
      of significant malaise, >/= 6 hours of sound sleep and abstinence from alcohol intake for at least 24 hr prior to the test. Participants were instructed to complete voiding
      and avoid food and drink for more than 3 hr before the scheduled test time. Pre-exercise measurements were taken in the sitting position after a 20-min rest. Subjects
      were monitored to pace their respiration at approximately 13 breaths/min.

      2.2. Method
      Heart Rate Variability (HRV) Analysis: By measuring heart rate and RR interval fluctuations (HRV), autonomic activity and the effects of mental balance and stress
      level on this capacity can be analogically inferred. RR interval is the distance between two consecutive R waves which are known as the largest spikes in EKG. Spectral
      transformation of this RR interval variability generates high frequency power called “HF” (> 0.15 Hz) and low frequency power termed “LF” (0.04 ~ 0.15 Hz), where
      HF power is regarded as the primary estimate of cardiac vagal activity. HF power is also generally presumed to correspond to respiratory sinus arrhythmia and therefore
      approximates to the frequency of respiration. In contrast, LF power is surmised as mirroring the activities of the two autonomic nerves. For these reasons, the ratio
      of HF and LF is considered as the index of sympathovagal activity. The LF/HF ratio is typically associated with sympathetic activation and HF with vagal excitation. These
      frequency domain indices are obtained from power spectral analysis that has been calculated from the fast Fourier transform (FFT) [10].
      Primary outcome parameters for this study were frequency domain parameters such as very low frequency (VLF), high frequency (HF), low frequency (LF), LF/HF
      ratio, total power (TP). Additionally, blood chemistry (AST, ALT, LDH, CK, blood glucose, BUN); differential WBC count; and blood lactate levels were obtained
      before and 10 min after all-out high knee lifts. HF Norm (adjusted HF) and LF Norm (adjusted LF) were used in lieu of HF and LF for statistical analyses.
      HF Norm = HF/(Total Power − VLF) × 100
      LF Norm = LF/(Total Power − VLF) × 100
      The purpose of adjusting the values was to minimize the impact of VLF alterations and to emphasize the separate fluctuations induced by parasympathetic and sympathetic
      activities. VLF has recently garnered attention as an important prognostic indicator of ischemic heart disease [11]. Although VLF is considered to reflect the
      very slow mechanistic activity of sympathetic control, the underlying physiological mechanisms remain unclear.
      Consequently, VLF measurements were omitted from this study and the adjusted values were used for statistical analysis.
      Based on the recommendation by The European Society of Cardiology and the North American Society of Pacing and Electrophysiology, resting HRV was measured
      with the Heart Rhythm Scanner (HRV analysis system from Biocom, Inc.) equipped with software that performs algorithms of short-term HRV analysis. Measurements
      were taken from subjects in sitting in a quiet room before the exercise. Biocom HRM-02 Pulse Wave Sensor, the photoplethysmography (PPG) monitor used
      in the study, was clipped to the right earlobe to measure HRV for 5 minutes. The same procedure was repeated after exercise. The PPG monitor was placed above the
      converter (light  energy) for the light source and photocells and adjacent to both earlobes to direct the infrared beam towards the photocells. Since blood volume
      fluctuations within the vasculature of the earlobe correlate to beat-to-beat changes, the PPG was employed to capture the pulse waves to generate data on HRV. At a
      designated area of a temperature (24℃) and humidity (60%) controlled testing room, participants performed two sets of 1-min all-out high knee lifts interspersed with
      1-min of passive recovery. Tests were conducted between 12:00 PM – 18:00 PM when HRV was least variable.
      In order to induce complete exhaustion, a supervising trainer provided verbal cues throughout the test period to monitor and ensure the pace of the exercise.
      Participants rested in standing during the 1-min interval and in supine for 10 minutes during post-exercise recovery.
      The same pre-exercise autonomic assessment and blood tests were repeated 10 min after the exercise to measure and quantitatively evaluate autonomic functions.
      Participants were instructed to maintain a consistent respiration rate for the autonomic assessment during recovery.
      Concomitantly with HRV analysis, routine blood tests for assessing conditioning level have been evaluated in this study, including: 1) blood chemistry aspartate
      amino transferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), creatine kinase (CK), blood urea nitrogen (BUN) to examine kidney
      functions and protein metabolism; 2) blood lactate concentration to assess glucose metabolism; and 3) differential WBC count to investigate recent appraisal of the correlation between sympathovagal activity and leukocyte subsets (neutrophils, lymphocytes, monocytes, eosinophils and basophils). Pre and post exercise blood samples were obtained from the same median cubital vein and sent to FALCO biosystems Ltd to be tested. Subjects were immediately asked about their fatigue rating after the exercise. All laboratory tests and physical measurements were taken while subjects sat calmly in the test room.
      Blood chemistry, differential WBC count, blood lactate level: The following measurements were taken before and after the stress exercise: 1) blood glucose (BG) to observe energy expenditure during exercise; 2) aspartate amino transferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), and creatine kinase
      (CK) to evaluate injury/inflammation of muscle tissues; 3) blood urea nitrogen (BUN) to assess alterations in kikidney functions and protein metabolism; 4) blood lactate
      concentration to determine glucose metabolism; and 5) differential WBC count (neutrophils, lymphocytes) to assess autonomic capacity.

      2.3. Statistical Analysis
      T-test with the level of significance set at P < 0.05 was used to assess pre and post exercise variations. All statistical analyses were carried out with PASW Statistics
      17.0 (SPSS Inc.) software.

      3. RESULTS
      3.1. HRV Analysis (Figures 1 - 5)

      The autonomic parameters of all subjects, including total power (TP), very low frequency (VLF), high frequency (HF), low frequency (LF) and LF/HF ratio during rest
      were all within normal limits.
      Figures 1-5 show the comparisons of mean TP, VLF, LF Norm, HF Norm and LF/HF ratio among the five sprinters.
      Comparisons of mean TP, VLF, LF Norm, HF Norm, and LF/HF ratio of the five sprinters before and 10 minute after maximal high knee lifts demonstrated a significant
      decrease in TP (p < 0.05), LF Norm(p < 0.01) and LF/HF ratio (p < 0.05), while a considerable increase in HF Norm (p < 0.05) was noted. VLF tended towards a
      decline despite statistical insignificance (p < 0.1).
       
      3.2. Blood Chemistry, Blood Lactate Concentration
      Blood chemistry and blood lactate level were all within normal limits before and after exercise. All aspects of blood chemistry of pre and post exercise samples did not demonstrate significant changes, except for creatine kinase (CK). Creatine kinase (CK) and blood lactate levels increased significantly 10 minutes after short, maximal
      exercise (p < 0.01).
       
      3.3. Differential Leukocyte Count (Figures 6 - 7)
      Figures 6-7 illustrate the comparisons of mean neutrophil
      and lymphocyte ratios among the five sprinters before and after all-out high knee lifts. The proportions of neutrophils and leukocytes in differential WBC count were all within normal limits. Neutrophils reduced significantly after exercise (p < 0.01), while lymphocytes
      increased significantly after exercise (p < 0.01).
       
      4. DISCUSSION
      A battery of blood tests, psychological assessments and autonomic evaluations are routinely administered to athletes to objectively quantify their conditioning level. The importance of autonomic capacity in athletic performance has been elucidated, and reports on autonomic evaluation using HRV analysis in athletes across various
      disciplines are available [12]. Despite recent accounts underpinning the correlation between autonomic activity and white blood cell count, data on this relationship in the field of sports and training are practically nonexistent.
      Although leukocytosis is a well-established phenomenon following physical activity, and while reports on differential WBC alterations in response to exercise have been published [13], the relationship between pre and post differential leukocyte and autonomic activity remains equivocal.
      The present study focused on quantitative evaluation of pre and post exercise autonomic activities as well as differential leukocyte count in athletes.
      Because the study targeted elite athletes who train regularly at high intensity and volume, all-out high knee lifts were selected as the exercise stress test to induce exhaustion. In the study, a prominent rise in peak lactate after maximal high knee lifts was noted which served as an objective marker of exhaustion in participants. Total
      power significantly decreased after exercise, but this was seen as a transient suppression of autonomic functions under substantial physical stress. Caution must be exercised when training athletes with reduced total power as this could indicate autonomic dystonia. The association between autonomic impairment and overtraining syndrome, a chronic state of fatigue, has been reported elsewhere.
      In the present study, significant post-exercise LF reduction was attributed to the inhibition of baroreceptor modulation in spite of sympathetic excitation after maximal exercise. In fact, LF appeared contingent not only on sympathetic tone but baroreceptor reflex as well.
      In response to a sudden increase in heart rate after short maximal workload, parasympathetic tone is thought to amplify 10 minutes after exercise to counteract the sympathetic surge. On this basis, post-exercise measurements for this study were taken 10 minutes after brief maximal load to assess changes during recovery. Modulation of acutely augmented heart rate after all-out exercise
      has far-reaching ramifications for athletes. The autonomic nervous system maintains physiological homeostasis during abrupt changes in physical states and is surmised to differ significantly between athletes and nonathletes.
      In general, it has been suggested that non-athletes have lower parasympathetic tone than athletes. Although this could not be substantiated from this study as data was obtained from high-performance athletes, athletes and non-athletes are nonetheless expected to demonstrate discrepant autonomic modulations after short maximal exercise.
      One sprinter was excluded from the study due to mental and physical exhaustion after participating in a 3- day collegiate track and field competition as the key player of his team. Resting autonomic tone of this athlete fell below the normal range and albeit pronounced subjective symptoms, the athlete failed to report his status prior to the beginning of the study. Moreover, continuous depression of parasympathetic activity was observed in this athlete. These results suggested that additional stress load on athletes suffering from overtraining syndrome could potentially prolong recovery. Despite reports of markedly higher vagal tone of athletes than nonathletes at rest, recovery after exercise also depends largely on this branch`s activity. In principle, cardiac autonomic
      assessment using HRV is analogous to the estimation of varying velocity of a moving vehicle from the coordination of the accelerator and brake. In other words, HRV is akin to the changing speed of a vehicle, while the operation of accelerator and brake corresponds to sympathovagal control of the heart. Because athletes must rapidly modulate an acutely elevated heart rate during
      rest, increasing the vagal tone has important implications.
      Our results indicated that LF/HF ratio, an index of sympathetic
      tone, following maximal high knee lifts declined, and that HF, an index of parasympathetic function, elevated 10 minutes after exercise. These results were consistent with a shift in proportion of neutrophils and lymphocytes in the differential leukocyte count, thus implying an association between autonomic functions and
      white blood cell count. In addition, these findings also undergirded the potential interactions between sympathetic activity and granulocytes, and parasympathetic activity and lymphocytes as cited by Abo [14]. With confirmed significant alterations in HRV parameters and differential leukocyte count in athletes following brief maximal exercise, quantitative evaluation of autonomic
      activity via HRV analysis and differential WBC emerge as plausible indices of conditioning level and physical capacity of athletes. The variance in neutrophil and lymphocyte ratios appears especially robust as an index, comparable to the assessment of autonomic functions.
      Participants of this study involved first-class athletes who were anticipated to demonstrate superior autonomic modulation following exercise. In the case of non-athletes, however, post-exercise recovery was expected to be more sluggish. Since our test date coincided with the track and field season, recruitment of robust number of top Japanese athletes for the study was difficult. Several
      participants withdrew from the study due to fatigue from regular training. The sample population fell below the target size as great care was taken to prevent the physical burden of participating in the study from ethically interfering with competitions and training programs. Moreover, the nature and intensity of the exercise stress test were such that only the very best athletes in the country
      could perform them adequately, and the selection of such athletes with comparable athletic capacity resulted in a maximum sample size of five for this study. Despite the small sample, this is the first study to evaluate autonomic resilience in sprinters after maximal exercise, and therefore we believe that our results can serve as a basis for continued exploration of autonomic modulations in
      athletes. While different results may have been observed had athletes from various disciplines participated in the study, sprinters were specifically selected because of their training emphasis on basic exercises and resulting ability to demonstrate rapid changes in HRV.
      According to our results, a greater sample size will minimize the individual differences in pre and post exercise parameters that were observed in this study. Additionally, future studies that provide a comparison with a control group and analyses of graded exercise intensity and post-exercise intervention will complement our study
      to deepen the understanding of the correlation between the autonomic nervous system and differential leukocyte count in athletes.

      REFERENCES
      [1] Hedelin, R., Wiklund, U., Bjerle, P., et al. (2000) Cardiac
      autonomic imbalance in an overtrained athlete. Medicine and Science in Sports and Exercise, 32(9), 1531-1533.
      [2] Hayano, J., Sakakibara, Y., Yamada, A. and Mukai, S.
      (1991) Accuracy of assessment of cardiac vagal tone by heart rate variability in normal subjects. American Journal of Cardiology, 67(2), 199-204.
      [3] Tanabe, S., Terao, T. and Nakano, S. (1993) Sympathovagal
      balance in young athletes. Tokai Journal of Sports Science and Medicine, 5, 44-49.
      [4] Tanabe, S., Terao, T. and Nakano, S. (1995) Comparison
      of sympathovagal balance between the middle aged and young athletes. Tokai Journal of Sports Science and Medicine, 7, 75-82.
      [5] Perini, R. and Veicsteinas, A. (2003) Heart rate variability
      and autonomic activity at rest and during exercise in
      various physiological conditions. European Journal of Applied Physiology, 90(3-4), 317-325.
      [6] Sawada, Y. (1999) Heart rate variability, Is it available in
      psychophysiological research. Japanese Journal of Cancer
      Research, 26, 8-13.
      [7] Yokoi, M. and Yamazaki, K. (1995) Cardiac autonomic
      nervous activity interaction under mental stress. A study
      using the corrected heart rate variability indexes. Tokai
      Journal of Sports Science and Medicine, 7, 75-82.
      [8] Abo, T. (2002) Autonomic nervous regulation of leukocytes.
      Japanese Journal of Physical Fitness and Sports
      Medicine, 51, 10-11.
      [9] Abo, T. (1999) Regulation of leukocytes by the autonomic
      nervous system, new rule on the cooperation of
      neuro-endocrine-immune systems (on the functions of
      the autonomic nervous system). Japanese Journal of
      Psychosimatic Medicine, 39, 67-74.
      [10] Goto, K., Matuura, H. and Muramoto, K. (2002) Estimate
      of autonomic nervous system function by heart rate
      variability analysis [in Japanese] IEICE technical report.
      ME and Bio Cybernetics, 102(507), 13-16.
      [11] Hadase, M., Azuma, A. and Zen, K. (2004) Very low
      frequency power of heart rate variability is a powerful
      predictor of clinical prognosis in patients with congestive
      heart failure. Japanese Circulation Society, 68(4), 343-
      347.
      [12] Tanabe, S., Yoshioka, K. and Yamashita, Y. (1994)
      Sympathetic and parasympathetic activities at rest and after exercise in judo athletes. Tokai Journal of Sports Science and Medicine, 6, 35-42.
      [13] Suzuki, K., Saoto, H. and Endo, T. (1995) Variation of
      neutrophil active oxygen and differential count of leukocyte’s
      during maximal exercise load in athletes. Japanese Journal of Physical Fitness and Sports Medicine, 44(6),
      747
      [14] Abo, T. (2002) Number and function of leukocytes are
      regulated by the autonomic nervous system. Journal of
      International Society of Life Information Science, 20(1), 171-189.
      上記論文は HEALTH Vol 2 No.10 に掲載されました。
      http://www.scirp.org/journal/health/

      Evaluation of autonomic nervous system by heart rate variability and differential count of leukocytes in athletes
      Nobuhiro Suetake, Yukiko Morita, Daichi Suzuki, Keiko Lee, Hiroyuki Kobayashi
      Abstract | References Full Paper: PDF , PP.1191-1198
      DOI: 10.4236/health.2010.210175

       
 

 

PRP療法によるアスリートケア

  • アキレス腱炎 ・ゴルフ肘  ・テニス肘
  • 肉離れ ・膝靭帯損傷 ・慢 性の筋肉の痛み
 ...など

トップアスリート株式会社のスポーツ医学・研究(PRP療法)

国内で初めてPRP療法によるアスリートへの治療は さかえクリニック(名古屋)で行われました。
国内初の臨床治療被験者は北京オリンピック前からアキレス腱周囲炎による強い痛みで競技に支障を来している アテネ・北京五輪陸上 110MH日本代表 内藤真人選手(ミズノ)。http://naitomasato.com/2010/05/

順調に復活を遂げました。
これまで三度の治療によりアキレス腱の痛みがほとんど改善し負荷のかかるトレーニングが十分に行えるようになりました。
2011年 世界陸上選手権、2012年のロンドン五輪を目指します。
欧米では広く行われつつある 再生医療治療法になります。
http://www.bloodcure.com/

オリンピック金メダリストも PRP療法で奇跡の復活を果たしました。
最近では、タイガーウッズ選 手がPRP療法で故障から回復したことをオフィシャルに
公表して 注目を集めました。

http://get- inthehole.com/player/tigerwoods/ tigerwoods_pressconf2_20100407.
html


国内では、アンチエイ ジング治療として美容分野へ導入され広く普及しております
が、 施術者の能力や使用するキットの質により効果も大きく異なる場 合がありま
す。
良質な血小板の採取と注入テクニックも結果に大きく影響を及ぼすと考えています。

当社では、提携医療機関の支援もあり 間違いなく 国内でも今後普及すると考えら
れる PRP療法のアスリートへの 施術を臨床実験・研究をスタートしました。
慎重にその経過と効果、手技 、注入量、注入部位、プロトコールを検討し、順天堂
大学医学部、さかえクリニック スポーツ診療部 の協力下、臨床研究を進めていき
ます。
国内では未だ、確立された治療法ではないため 被験者は限定されますが、痛みや故
障で苦しむアスリートに微力ながらお役にたてれば幸いです。

 
 
 

現在、実験ボランティア募集中です。

以下の条件に該当されるアスリートを募集しています。(倫理 的な条項、研究にあたっての詳細を説明させていただきます。)

費用はすべて無料となります。( 当社へお越しになられる際の交通費はご負担下さい。)

  • オリンピック日本代表(競技 種目は問いません)
  • 世界選手権およびワールドカ ップでメダルを獲得された選手
  • プロ野球選手で1軍の経験者
  • プロアスリートで日本ランキ ング1位(格闘技は除く)

ただし、順天堂大学医学部付属病院で 画像診断、診察を受けていただくこと、定期的な検査、治療部位 の状態の定期的報告を義務とさせていただきます。
何卒、よろしくお願いいたします。

 

緊急レポート

アンチエイジングシステム エアナジーが放射線障害に有効か

放射性障害にも有効の報告(ウクライナのチェルノブイリ地区での放射能汚染による健康被害の治療にエアナジーが使用されています。)
エアナジーによる一重項酸素療法は、福島原発事故での放射能汚染のケアに役立つ可能性がドイツのメーカーよりアナウンスされました。


Report on clinical investigations of Singlet Oxygen Therapy. SOE respiratory unit use in sanitarium practice at Chernobyl zone in Ukraine.

Abbreviations in this study are:
SOT = Singlet Oxygen Therapy
SOE = Singlet Oxygen Energy
Introduction.
SOT is widely practiced among the new perspective methods on non-medicamental treatment and prophylactics of diseases.
The principle of the SOE (Singlet Oxygen Energy)-effect is based on optimization of singlet oxygen formation using special AIRNERGY catalysts in an activation chamber of a respiratory unit and its delivery into a human body.
Normalization of the patients’ health condition after the Airnergy SOE-therapy was caused by improvement of blood circulation, stimulation of metabolism in tissues, immunity strengthening, detoxification, normalization of antioxidant state. To put SOE into practice, modern Valkion Unit, including the AIRNERGY catalysts, was set in dispensary “Teteriv”, Korostyshisky district of Zhytomyr region where the above mentioned investigations have been fulfilled since the 1st of July 1997 till the 23rd of February 1999.
Research Object and Task.
The aim of the present investigation was to determinate the SOE-therapy influence on health condition of patients in sanitarium, and possibilities and perspectives of SOT use for the treatment and rehabilitations of patients with different types of pathologies.
To fulfill the purpose the following tasks were set:
• study the effectiveness of SOE use in clinical practice based on treatment results of the patients with different types of diseases.
• organize the comparative characteristics of SOE effectiveness among the patients who received SOE-therapy in complex with other types of treatment and those who didn’t receive SOE at all.
• make the conclusion on SOE effectiveness and use perspectives in sanitarium practice on the basis of clinical and laboratory data.
The treatment by means of SOT consisted of energized water use and activated air inhalation. Patients received SOE 1 time a day on an average. During the first day of treatment patient consumed 50-100 ml of activated water and 3-9 minutes of inhalation by activated air. The second and all the following days, patients took 150-200 ml of activated water and 6 – 14 min. of inhalation. Each patient received 11 SOE treatments on average.
Results of the Investigation.
When using SOE, i.e. Singlet Oxygen Energy, in complex with other types of treatment (please refer to the table in Attachment 1), all the children (455 persons) finished their course of treatment with improvement of their condition. In the control group only 451 out of 512 children (88,10 %) finished their treatment with improvement. 58 patients (11,32 % left without changes, 3 patients aggravated their stage.
When treating the patients with blood circulation system diseases, it was noted, that general effectiveness of SOE use in the first group amounted to 100 %.
The effectiveness of SOT by the patients with blood circulation system pathologies was caused by improvement of electrocardiogram indices, increase in patients’ tolerance to the physical load, disappearance of heartache and tachycardia, increase of hemoglobin level in blood. SOE-effectiveness in the control group amounted to 91,06 %. Out of 302 children 275 persons felt better, 25 patients didn’t change their health condition, 2 children finished their course of treatment with aggravation.
The SOE therapy applied to 112 children suffering from respiratory tract diseases showed considerable effectiveness.
There were several changes for better which are observed by all the patients disappearance of catarrhal phenomenon, facilitation of expectoration, stabilization of external breathing function, reduction of short breath and quantity of choke attacks by the patients who suffered from bronchial asthma. The treatment effectiveness of the patients from the control group was 84,13 % out of 126 children 106 improved their health, 19 children (15,1 %) finished the course of treatment without changes. 1 child aggravated the health condition.
The effectiveness of the SOE-therapy applied to 31 children with gastrointestinal tract diseases was characterized by the remarkable improvement of health condition of all the patients. After SOE treatment patients’ health condition improved, pains in stomach disappeared, general state and appetite improved, meteorism disappeared, heartburn reduced, stool normalized. The SOE effectiveness in the control group amounted to 70,97 %, 9 patients (29,09 %) left without changes.
All the patients from the first group who had metabolism pathologies finished their course of treatment with improvement of their health condition. There were following changes in health condition of the patients: disappearance of thirst and dryness in month, tiredness, reduction of sugar in blood of the patients who suffered from sugar diabetes, and loss of weight in case of obesity. By comparison, in the control group 85,71 % of patients improved their health.

Materials and methods of investigation.
967 children (aged 6 – 15) from the region polluted by radiation were treated by means of SOE in dispensary Teteriv.
The experimental persons were divided into 2 groups. In the first group there were 455 children, who underwent complex treatment (SOT in complex with traditional treatment at the same time). Among them: 57,4 % suffered from diseases of blood circulation system, 24,6 % from respiratory tract pathologies.
There was following percentage according to the level of affection:
Light – 54,2 %, middle-hard – 42,3 %, hard – 3,5 %.
The second (control) group consisted of 512 children, who were treated only by means of the SOE-therapy. The overwhelming majority of patient suffered from blood circulation system diseases (59,0 %) and respiratory tract diseases (24,6 %. 55,8 % of children had light level of affection, 39,6 % - middle-hard level, 4,7 % - hard.
In most cases the patients with cardio-vascular system pathologies suffered from the following diseases:
• functional cardiopathy,
• vegetovascular distony.
Among gastrotestinal tract diseases prevailed:
• chronic gastritis,
• gastro-duodenitis,
• cholecystitis,
• diskinesia of bile duct.
Among respiratory tract diseases predominated:
• chronic and obstructive bronchitis,
• bronchial asthma
Among metabolism pathologies prevailed:
• endocrine system diseases including sugar diabetes (of light and middle-hard form).
Among motor system diseases prevailed:
• after-traumatic bones and joints injury.

Among skin diseases prevailed:
• eczema,
• neurodermatitis
For the investigation of SOE therapeutic effectiveness different data was studied: data of electrocardiogram, indices of coagulogram, hemoglobin level, sugar in blood (prothrombin index, fibrinogen), the patients’ tolerance to physical load was also taken into consideration.
The possible side effect was evaluated by means of the following symptoms:
• appearance of pain of different localization, which didn’t bother patient before,
• intensification of disease symptoms which patient had before SOE,
• appearance or intensification of neurological stage
• worsening of clinic-functional and laboratory indices (if they were not caused by medicament therapy).
There was 100 % effectiveness after SOE energy therapy by the patients with nervous system diseases, in the control group 85,71 % of patients became better.
The SOE effectiveness by the patients who suffered from skin and motor system diseases was high but didn’t differ much from the results in the control group.
All the 16 persons who suffered from kidney and urinary tract diseases and to whom Singlet Oxygen Therapy in complex with other methods of treatment was applied changed their health condition for better, in the control group 91,67 % of patients improved their health.
Analysis on the results of SOE use applied to the children living in the territory polluted after accident at Chernobyl Atomic Power Station and treating in the dispensary “Teteriv” showed considerable treatment effectiveness. Practically 100 % of patients felt disappearance of headache and also pain in joints and in chest region, improvement of general condition, increase in weight, disappearance of nasal bleeding and catarrhal phenomenon in gullet, normalization of blood composition and decrease in caesium-137 level.
1. Research work on Singlet Oxygen Energy Therapy use in sanitarium practice of dispensary “Teteriv” shows a remarkable effectiveness for the patients who underwent SOT in complex with other methods of treatment.
2. Effectiveness of the SOE-method in the first group (SOE-therapy in complex with traditional treatment) was 11,9 % higher by comparison with the control group.
3. Analysis on the results of the clinic research shows expediency and profit SOE use in sanitarium practice of Ukraine treating the children with different types of pathologies from the ecologically unfavorable regions and areas polluted by radiation.

Closing Statement Concerning Copyright
This article was published in 1999 by POLYVALK AB, Att. Antony van der Valk, Göteborg, Sweden. Respecting the Copyright of the study, it should be noticed that the study investigations were performed with Valkion units that were equipped with AIRNERGY+ catalysts! As the catalysts are the key of the method, AIRNERGY AG headquartered at Hennef, Germany, puts forward a certain claim on these published data as well!









Attachment 1: Table
Attachment 2: Statement of AIRNERGY Medical Management



Attachment 1: Effectiveness of Singlet Oxygen Energy Therapy Use according to the Type
of the main Pathology in Sanatoria of Teteriv

Kind of the main pathology Patients who undergone SOET and other kinds of treatment (I group) Treatment effective-ness, % Patients who didn´t received SOET Treatment effective-ness, %
Total With improve-ment Without changes With aggravation Total With improvement Without changes With aggravation

Blood circulation organs 261 261 100 302 275 25 2 91,06
Gastrointestinal tract 31 31 100 31 22 9 70,97
Metabolism 7 7 100 7 6 1 85,71
Nervous system 7 7 100 12 10 2 83,33
Motor functions 9 9 100 12 11 1 91,67
Respiratory tract 112 112 100 126 106 19 1 84,13
Women´s disease 100
Skin 12 12 100 10 10 100
Kidney and urinary tract 16 16 100 12 11 1 91,67

TOTAL 455 455 100 512 451 58 3 88,69

Attachment 2: Statement of AIRNERGY Medical Management
With the broad and terrible experiences after the atomic fallout in Chernobyl, human mankind should have learned that an immediate and an overall prevention of immediate or subsequent events caused by the atomic pollution are out of the question. The (governmental) installation of an overall prevention concept is of major importance, especially regarding the population´s fears and helplessness con-cerning their current and future health status. This is true for the generation who witnessed the catastrophe, but also for those generations to come.
Based on the experiences of Chernobyl we have to come to the conclusion, that it is inalienable to start an overall preventive therapy immediately. This early treatment approach can help to avoid further cell damage in the living population, and might be also effective in reducing a possible harm for the following generations.
Therefore, and based on the results of the above publication it is absolutely out of the question to start a prevention/ supportive therapy in the population that are directly threatened by the atomic catastrophe, not only in the central region of Fukushima, but also in all possibly polluted areas in Japan, immediately!
The inhalative Singlet Oxygen Energy Therapy (SOET) should be combined with drinking of the activated water. Combining the two different routes of administration shows a synergistic effect (by a complete coverage of the entire body including the respiratory tract and the gastrointestinal passage) and supports to attain a maximum effect in the body. This combination should have the greatest and a long-term effect on the patients prevention of further cellular damage and an immediate reduction of cancerogenous products (for example, as reported in the above article, a decrease in caesium-137 levels*).
From the AIRNERGY+ point of view the respiratory unit Basis Plus should be sufficient as the catalysts show a much greater energy expansion, than they did years ago, a result of AIRNERY´s + former market leader position and the continuous improvement of the SOE catalyst development processes.
People affected, or people concerned to be or to become affected should breathe the SOEnergy once or two times daily for approximately 40 minutes and drink the energized water after the end of the breathing session. Please recognize, that the water used for the inhalator needs to have drinking water quality.
There is a second version of the AIRNERGY+ inhalation system available – called Professional Plus. As this version contains more catalysts, the breathing time can be reduced to 21 - 30 minutes.
Both product versions have the same mode of action. The major difference is, that recognizable effects can be perceived earlier and the maximum of biological effectiveness is higher using the Professional Plus than with the Basis Plus product.
Although the above mentioned study was only performed in children, AIRNERGY+ does not expect any different results in grown-ups, taking a more than 10-year experience with both populations into account.
It should be an immediate task to get in contact with hospitals, doctors and other medical or paramedical institutions. In addition a press release might be helpful to give the population, which feels helpless now, an alternative and a strong support in their fight against this unknown enemy – radioactive pollution.
In my personal opinion, the government and in special the Japanese Ministry of Health should be informed about AIRNERGY+ as a preventive, therapeutic and curative option that can help to stabilize and recover their population. It is easily to use and without any side effects. The installation of free governmental breathing stations might be a fundamental idea. Managed and controlled by a medically trained person, people can go there to take their breathing/drinking therapy and are under medical care as well. This approach might strengthen the feeling that the government takes care of each single individual!
To help the Japanese Population in this outstanding catastrophe AIRNERGY+ will support any activities to make AIRNERGY+ available for the Japanese population as easily and as soon as possible!

 


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